evaluation of morphology and cell behaviour of a novel synthesized electrospun poly(vinyl pyrrolidone)/poly(vinyl alcohol)/hydroxyapatite nanofibers

objective(s): three-dimensional structures such as nanofibrous scaffolds are being used in biomedical engineering as well as provide a site for cells to attach and proliferate leading to tissue formation. in the present study, poly(vinyl pyrrolidone) (pvp)/ poly(vinyl alcohol)(pva) hybrid nanofibrous scaffold was synthesized by electrospinning. materials and methods: the effect of adding nano hydroxyapatite (n-ha) and also epoxypropoxy-propyl-trimethoxysilane (epptms) as a crosslinking agent on morphology and cell behaviour of the nanofibers was investigated.results: scanning electron microscope (sem) observations showed that all kinds of nanofibers represented uniform and well-ordered morphologies without formation of any beads by controlling the synthesis parameters. the average ûber diameter of pvp-pva was 260 nm. n-ha was synthesized by wet chemical process. the synthesized n-ha was characterized by xrd for structural analysis. transmission electron microscope (tem) revealed that ha particles had nanosized dimensions (in the range of 40-100 nm). the presence of n-ha within electrospun pvp-pva nanofibers was confirmed by xrd and fourier transmission infrared spectroscopy (ftir) analyses. the average ûber diameter was decreased to 136 nm when n-ha was added in the composition of pvp-pva. ftir analysis depicted that pvp-pva nanofibers were linked to epptms as a biocompatible material by the covalent bond. although adding n-ha caused to decrease the diameter of pvp-pva nanofibers, addition of epptms within pvp/pva/n-ha nanofibers induced increasing distribution of fiber diameter as it enhanced to 195nm. in addition, the proper proliferation of g292 osteoblastic cells without any cytotoxicity was observed for the nanofiber.conclusion: since these materials have been known as biomaterials, pvp/pva/n-ha-epptms nanofibers can be propounded as a good candidate for bone tissue engineering application.